RT ProfilerTM PCR Arrays: Pathway-Focused Gene Expression Profiling with qRT-PCR
نویسندگان
چکیده
This paper evaluates the performance of the newest technique for monitoring the expression of a panel of pathwayor disease-specific genes: the RT2 Profiler PCR Array System from SA Biosciences. The PCR Array System combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray. The PCR Array is a 96or 384-well plate containing RT2 qPCR Primer Assays for a set of 84 related genes, plus five housekeeping genes, and three controls. A complete system includes an instrumentspecific master mix and an optimized first strand synthesis kit. This paper presents scientific data showing that PCR Arrays have the sensitivity, reproducibility, and specificity expected from real-time PCR techniques. As a result, this technology brings focused gene expression profiling to any biological laboratory setting with a real-time PCR instrument.
منابع مشابه
Semiquantitative RT-PCR analysis to assess the expression levels of iNOS in chicken macrophages
Abstract: iNOS is inducible by a variety of factors related to inflammation and referred to as inducible NOS(iNOS). It is regulated at the level of gene expression; once expressed, it produces NO at a high rate. iNOS gene-expression profiling is an important tool in understanding molecular markers of the responses of cells and tissues to external factors. In this article a semiquantitative reve...
متن کاملUBE2Q1, as Down Regulated Gene in Pediatric Acute Lymphoblastic Leukemia
Ubiquitin - proteasome system (UPS), the major protein degradation pathway in the cells, typically degrades short - lived and damaged proteins and regulates growth and stress responses. This pathway is altered in various cancers, including Acute Lymphoblastic Leukemia (ALL). ALL begins with a change in bone marrow cells and is the most common type of leukemia in children under 15 years. UBE2Q1...
متن کاملNormalization of microRNA quantitative RT-PCR data in reduced scale experimental designs.
Proper normalization of quantitative RT-PCR (qRT-PCR) data is a crucial component of gene -expression analysis. Although arbitrarily selected housekeeping genes have been used to normalize many published mRNA RT-PCR datasets, there is a growing awareness that such normalizers should be first validated empirically. The use of stable reference genes is particularly needed for qRT-PCR of microRNA ...
متن کاملEffect of valproic acid on JAK/STAT pathway, SOCS1, SOCS3, Bcl-xL, c-Myc, and Mcl-1 gene expression, cell growth inhibition and apoptosis induction in human colon cancer HT29 cell line.
Background and aim: Cytokines are a large family of protein messengers. These proteins induce various cellular responses. Janus kinases (JAKs) are mediators of cytokine, activated JAKs phosphorylate signal transducers, and activators of transcription (STAT) proteins that regulate cell differentiation, proliferation, and apoptosis. Aberrant JAK/STAT signaling is involved in the oncogenesis of se...
متن کاملUse of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies.
Real-time reverse transcription polymerase chain reaction (RT-PCR) methods that monitor product accumulation were adapted for the validation of differentially expressed genes. We describe a real-time quantitative PCR assay that uses SYBR Green I dye-based detection and product melting curve analysis to validate differentially expressed genes identified by gene expression profiling technologies....
متن کامل